High Performance Liquid Chromatography (HPLC)

About this course

High Performance Liquid Chromatography (HPLC) techniques are so popular and common in pharma industry, cosmetic industry, chemistry industry, food industry, polymer industry and environment laboratories.High performance liquid chromatography (HPLC) is the crucial and integral analytical tool applied in all stages of drug discovery, development, and commercialization in the pharmaceutical industry, especially. At the each step of drug development, drug substances, ingredients and drug products are controlled and monitored by using HPLC techniques.In this course, chromotography, chromatography principles, types of chromatography, instrumental design of HPLC, HPLC Analysis, Types of HPLC and Descriptors of HPLC have been discussed within all technical details.HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) COURSE SCOPE1 INTRODUCTION 2 INTRODUCTION TO HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) 3 CHROMATOGRAPHY 3_1 History of Chromatography 3_2 What is Chromatography? 3_3 What is Stationary Phase? 3_4 What is Mobile Phase? 3_5 What is Analyte? 4 CHROMATOGRAPHY PRINCIPLES 4_1 Chromatographic Seperation 4_2 Chromatography (Affinity & Interaction) 4_3 Chromatography (Solubility & Adsorption) 4_4 Chromatography (Solubility & Polarity) 4_5 Chromatography (Solubility & Hidrophilic / Hidrophobic) 4_6 Let's Categorize Molecules! 5 TYPES OF CHROMATOGRAPHY 5_1 Chromatography Classification Based on Bed (Column Chromatography, Paper Chromatogrphy, Thin Layer Chromatography) 5_2 Chromatography Classification Based on Separation Mechanism (Affinity Chromatography, Ion Exchange Chromatography, Gel Filtration Chromatography) 5_3 Chromatography Classification Based on Physical States of Phases (Gas Chromatography